EXTERACELLULAR L-ASPARAGINASE FROM LOCAL SOIL BACTERIA: PRODUCTION AND OPTIMIZATION OF PHYSIOLOGICAL CONDITION

Authors

Hanaa Orabi, Botany & Microbiology Department, Faculty of Science, Al-Azhar University (Girls Branch)
Esmail El-Fakrany, Protein Research Department, Genetic Engineering and Biotechnology Research Institute, City for Scientific Research and Technology Applications, Alexandria, Egypt.
Eman Abdel Khalek, Botany & Microbiology Department, Faculty of Science, Al-Azhar University (Girls Branch)
Aziza El-Kasaby, Botany & Microbiology Department, Faculty of Science, Al-Azhar University (Girls Branch)
Nagwa Sidkey, Botany & Microbiology Department, Faculty of Science, Al-Azhar University (Girls Branch)

Corresponding Author

Orabi, Hanaa

Document Type

Original Article

Subject Areas

Botany, Microbiology and Zoology

Keywords

L-asparaginase; enzyme production; submerged fermentation and solid state fermentation

Abstract

L-asparaginase produced by Bacillushalotolerans OHEM18 finds broad applications due to its hydrolysis activity so used as therapeutic agents in combination with additional medications in the cure of acute lymphoblastic leukemia and also in food processing. In the present study, L-asparaginase production was optimizedand the optimum factors for production under submerged fermentation (SMF) of the asparaginase were pH 7.0 after 28h using glucose and asparagine as carbon and nitrogen sources, respectively while bagasse was the best inducer for L-asparaginase production under SSF.

How to Cite This Article

Orabi, Hanaa; El-Fakrany, Esmail; Abdel Khalek, Eman; El-Kasaby, Aziza; and Sidkey, Nagwa (2018) "EXTERACELLULAR L-ASPARAGINASE FROM LOCAL SOIL BACTERIA: PRODUCTION AND OPTIMIZATION OF PHYSIOLOGICAL CONDITION," Al-Azhar Bulletin of Science: Vol. 29: Iss. 2, Article 2.
DOI: https://doi.org/10.21608/absb.2018.33806