PRODUCTION OF AN EXTRACELLULAR HALOPHILIC AMYLASE FROM THE EXTREMELY HALOPHILIC ARCHAEON NATRIALBA AEGYPTIACA STRAIN 40T

Authors

NOURA HAGAGGI, Botany Department, Faculty of Science, Aswan University, Aswan, Egypt
FRANCIS HEZAYEN, Botany Department, Faculty of Science, Aswan University, Aswan, Egypt
USAMA ABDULRAOUF, Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Assiut, Egypt

Corresponding Author

ABDULRAOUF, USAMA

Document Type

Original Article

Subject Areas

Botany, Microbiology and Zoology

Keywords

halophilic; Natrialba aegyptiaca; amylase; production; Optimization; raw starches

Abstract

The extremely halophilic archaeon, Natrialba aegyptiaca strain 40T, which was isolated from a salty soil close to Aswan, Egypt is able to produce a halophilic extracellular, raw starch-digesting amylase. Optimization of medium components and culture conditions to enhance amylase production was investigated. Maximum production of this amylase was achieved in a medium contained (%, w/v): NaCl, 15; KCl, 1.5; MgSO4.7H2O, 0.1 and soluble starch, 0.05 at a pH range between 6-8 and a temperature range between 45-47 oC after incubation period of 144 hr. under static conditions and 48 hr. with shaking at 150 rpm. The enzyme could efficiently hydrolyze raw starches from different plant sources.

How to Cite This Article

HAGAGGI, NOURA; HEZAYEN, FRANCIS; and ABDULRAOUF, USAMA (2013) "PRODUCTION OF AN EXTRACELLULAR HALOPHILIC AMYLASE FROM THE EXTREMELY HALOPHILIC ARCHAEON NATRIALBA AEGYPTIACA STRAIN 40T," Al-Azhar Bulletin of Science: Vol. 24: Iss. 2, Article 19.
DOI: https://doi.org/10.21608/absb.2013.6422