STUDIES ON THE INDUCTION OF LIPASE(S) BY THERMOALKALOPHILIC BACILLUS bREVIS B2

Authors

EGLAL GHONEIMY, Botany and Microbiology Department, Faculty of Science (Girls), Al-Azhar University, Cairo, Egypt.
REDA BAYOUMI, Botany and Microbiology Department, Faculty of Science (Boys), Al-Azhar University, Cairo, Egypt.
SANAA AHMED, Botany and Microbiology Department, Faculty of Science (Girls), Al-Azhar University, Cairo, Egypt.
NAHID YOUNIS, Nuclear Research Center, Atomic Energy Authority, Egypt.
OLA EL-HAGER, Nuclear Research Center, Atomic Energy Authority, Egypt.

Corresponding Author

GHONEIMY, EGLAL

Document Type

Original Article

Subject Areas

Botany, Microbiology and Zoology

Keywords

Thermoalkalophilic bacteria; Lipase; physiology

Abstract

Bacillus brevis-B2 was selected as the most patent lipase(s) producer among nine thermoalkalophilic bacterial isolates isolated from agro industrial wastes at 70oC and pH 10.5. Of the tested nutrient medium, tap water containing 1% fish wastes and 1% yeast extract is the best inducer for lipase synthesis by B. brevis B2. Maximum enzyme biosynthesis could be obtained after 24 hr of incubation. The optimal pH and temperature for enzyme formation are 11.5 and 70oC. Enzyme yield increased in presence of sucrose or lactose in the nutrient medium, also the presence of 100 ppm zinc sulphate and 500 ppm folic acid supported high induction of lipase by B. brevis growing under shaking condition and inocula size of 2ml/100ml culture medium. A very slight increase in enzyme formation occur under exposure of bacterial isolate to 1 KGy of γ-irradiation.

How to Cite This Article

GHONEIMY, EGLAL; BAYOUMI, REDA; AHMED, SANAA; YOUNIS, NAHID; and EL-HAGER, OLA (2009) "STUDIES ON THE INDUCTION OF LIPASE(S) BY THERMOALKALOPHILIC BACILLUS bREVIS B2," Al-Azhar Bulletin of Science: Vol. 20: Iss. 2, Article 1.
DOI: https://doi.org/10.21608/absb.2009.7561